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相关概念视频

Bioplastics01:27

Bioplastics

Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
Microbial Bioremediation of Plastics01:28

Microbial Bioremediation of Plastics

Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...
Hydrolysis01:15

Hydrolysis

Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...

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Designed for Molecular Recycling: A Lignin-Derived Semi-aromatic Biobased Polymer
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Designed for Molecular Recycling: A Lignin-Derived Semi-aromatic Biobased Polymer

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可回收的热敏聚合物-细胞酶生物结合物用于生物质脱聚合.

Katherine J Mackenzie1, Matthew B Francis

  • 1Department of Chemistry, University of California, Berkeley, and Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720-1460, United States.

Journal of the American Chemical Society
|December 29, 2012
PubMed
概括

研究人员开发了一种可重复使用的聚合物-内葡萄糖酶生物结合物,具有可调节的热敏性质. 这种酶结合物对纤维素保持高活性,使生物质转化和酶回收能够有效地用于可持续的工业应用.

科学领域:

  • 生物结合化学 生物结合化学
  • 聚合物科学 聚合物科学
  • 酶工程是什么? 酶工程是什么?

背景情况:

  • 开发可回收和可重复使用的酶系统对于具有成本效益的工业生物催化剂至关重要.
  • 热敏聚合物提供了基于温度变化的酶固定和分离的潜力.
  • 内葡萄糖酶是分解纤维素成可发酵糖的关键酶.

研究的目的:

  • 构建和描述一种具有可调节性质的热敏聚合物-内葡萄糖酶生物结合物.
  • 评估生物结合物的活性,稳定性和可重复使用性在不溶性纤维素和纤维素基生物质上.
  • 展示一种用于生物质转换的酶聚合物联合体的多功能策略.

主要方法:

  • 合成含有氨基氧的共聚合物,可调节较低的临界溶液温度 (LCST).
  • 通过转胺和氧胺结合,对内葡萄糖酶 (EGPh) 的选择性修饰.
  • 聚合物-内葡萄糖酶生物结合物的构建及其酶活性和回收的表征.

主要成果:

  • 生物结合物表现出类似于不溶性纤维素上未经修改的内葡萄糖酶的酶活性.
  • 在两次重复使用周期后,NIPAm共聚物-EGPh合物保持了超过60%的活性.
  • 在三轮的生物结合物治疗中,Miscanthus的降低糖产量增加了2.8倍.

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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

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Published on: November 30, 2020

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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

结论:

  • 成功开发了一种可回收,热敏的聚合物-内葡萄糖酶生物结合物.
  • 可调节的LCST和多功能合策略使其在酶固定和生物质处理方面具有广泛的应用.
  • 这种方法促进了纤维素材料的高效和可持续的酶转化.