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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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相关实验视频

Updated: May 15, 2026

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

使用CRISPR/Cas系统进行多重基因组工程.

Le Cong1, F Ann Ran, David Cox

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|January 5, 2013
PubMed
概括
此摘要是机器生成的。

这项研究引入了CRISPR-Cas9基因编辑,用于在人类和小鼠细胞中精确地切割DNA. 这项技术可以同时编辑多个基因组站点,为遗传研究提供广泛的适用性.

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

相关实验视频

Last Updated: May 15, 2026

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物技术是生物技术.

背景情况:

  • 精确的基因组编辑对于理解遗传变异至关重要.
  • Prokaryotic 的 CRISPR/Cas 系统提供了 RNA 指导的 DNA 分裂能力.

研究的目的:

  • 设计和证明CRISPR-Cas9系统在哺乳动物细胞中进行向基因组编辑的有效性.
  • 探索RNA引导核酶的可编程性和适用性,用于功能基因组学.

主要方法:

  • 两个不同的II型CRISPR/Cas系统的工程.
  • 使用由短RNA指导的Cas9核酶进行特定地点的DNA裂变.
  • 将Cas9转化为一个nicking酶,用于同质导向的修复.

主要成果:

  • 通过使用工程CRISPR-Cas9系统,在人类和小鼠细胞内源性基因组位点进行了精确的切割.
  • 展示了最小的突变性活性,具有入Cas9变体,用于增强修复.
  • 通过单个CRISPR阵列实现了同时多站点的基因组编辑,突出了可编程性.

结论:

  • 工程RNA引导的CRISPR-Cas9核酶技术具有高度可编程性,并且广泛适用于哺乳动物基因组编辑.
  • 该系统能够以精确和高效的方法对遗传变异和元素进行功能性阐明.