人类一般转录因子TFIID核心复合体的架构
Christoph Bieniossek1, Gabor Papai, Christiane Schaffitzel
1European Molecular Biology Laboratory Grenoble Outstation, Unit of Virus Host Cell Interactions UVHCI, UJF-CNRS-EMBL Unité Mixte International UMI 3265, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France.
Nature
|January 8, 2013
概括
人类核心TFIID的结构显示了一个对称的架构,在TAF8-TAF10结合时转变为不对称,指导holo-TFIID组装用于基因转录调节.
科学领域:
- 分子生物学分子生物学
- 结构生物学 结构生物学
- 基因规则 基因规则
背景情况:
- 转录因子IID (TFIID) 对于由RNA聚合酶II启动基因转录至关重要.
- TFIID是一个大型的TATA盒结合蛋白 (TBP) 和13个TBP相关因子 (TAF) 综合体.
- TFIID的精确架构和组装路径在很大程度上是未知的.
研究的目的:
- 为了确定人类核心的高分辨率结构-TFIID.
- 阐明核心TFIID对称性在全光TFIID组装中的作用.
- 了解TFIID如何促进基因转录.
主要方法:
- 低温电子显微镜 (cryo-EM) 的分辨率为11.6 Å.
- 人类核心的结构分析-TFIID.
- 生物化学试验研究TAF8-TAF10复合物的结合.
主要成果:
- 人类核心-TFIID的结构呈现出双重对称,交错的架构.
- 在核心-TFIID结构中,TAFs的保存结构特征,包括基因组折叠,都被容纳在内.
- TAF8-TAF10复合物的结合破坏了核心-TFIID的对称性,形成了一个不对称的支架.
结论:
- 非对称的核心-TFIID结构作为一个核化场所,用于全体TFIID组装.
- 这种从对称到不对称的结构过渡对于TFIID组装路径至关重要.
- 了解TFIID结构,可以了解基因转录的调节.
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