从诱导多能或胚胎干细胞中衍生出的终端分化细胞的免疫性可以忽略不计
Ryoko Araki1, Masahiro Uda, Yuko Hoki
1Transcriptome Research Group, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
Nature
|January 11, 2013
概括
诱导多能干细胞 (iPSC) 和胚胎干细胞 (ES) 在分化成用于移植的皮肤和骨髓组织时表现出类似的低免疫性. 这表明iPSC衍生组织对于再生医学是安全的,从而最大限度地减少了免疫排斥的担忧.
科学领域:
- 干细胞生物学 干细胞生物学
- 免疫学 免疫学 免疫学
- 再生医学是一种再生医学.
背景情况:
- 胚胎干细胞 (ES) 在再生医学中面临着伦理问题和免疫排斥风险.
- 诱导多能干细胞 (iPSC) 提供了一个替代方案,但由于部分重编程和遗传不稳定性,它们的潜在免疫性需要仔细评估.
- 之前的研究对iPSC衍生组织的免疫性产生了相互矛盾的结果.
研究的目的:
- 调查和比较从小鼠iPSCs与ES细胞衍生出的分化皮肤和骨髓组织的免疫性.
- 评估iPSC和ES细胞衍生组织移植后免疫反应的潜力,包括T细胞透.
- 检查涉及iPSC免疫性的一些特定基因 (Zg16,Hormad1) 的表达.
主要方法:
- 从C57BL/6小鼠中建立了10个无集成iPSC线和7个ES细胞线.
- 在体外将差异化的iPSC和ES细胞转化为皮肤和骨髓组织.
- 进行了移植实验,比较iPSC衍生的和ES细胞衍生的组织.
- 分析了免疫反应的组织,包括T细胞透和基因表达.
主要成果:
- 在iPSC衍生的皮肤和骨髓组织和ES细胞衍生的皮肤和骨髓组织之间,在移植成功率上没有发现显著差异.
- 在来自iPSC或ES细胞的组织中检测到有限或没有免疫反应,例如T细胞透.
- 在回归的皮肤和瘤组织中没有发现Zg16和Hormad1基因的增加表达.
结论:
- 从iPSC和ES细胞中分化的移植细胞具有有限的免疫性.
- 从iPSC衍生的组织在免疫排斥方面表现出与ES细胞衍生的组织相似的安全概况.
- 这些发现支持iPSC衍生的分化组织在再生医学中的潜在使用,减少了对免疫反应的担忧.
更多相关视频
06:24Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells
Published on: October 20, 2023
12:43Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
Published on: December 6, 2013
相关概念视频
01:06Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Somatic cells are...
01:13Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
01:13Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
01:17Stem Cell Culture
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
01:27Source And Potency Of Stem Cells
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...