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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
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由人工小分子机器进行序列特异性合成.

Bartosz Lewandowski1, Guillaume De Bo, John W Ward

  • 1School of Chemistry, University of Manchester, Oxford Road, Manchester M13 9PL, UK.

Science (New York, N.Y.)
|January 12, 2013
PubMed
概括

研究人员设计了一种新型的人工分子机器,可以用特定序列合成. 这种基于罗他森的机器沿着一个链移动,添加氨基酸制造,模仿生物蛋白质合成.

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科学领域:

  • 分子生物学分子生物学
  • 合成化学 合成化学
  • 纳米技术 纳米技术

背景情况:

  • 核糖体是天然的分子机器,负责基于信使RNA序列的蛋白质合成.
  • 目前用于合成的方法可能很复杂,可能缺乏生物系统的精度.

研究的目的:

  • 设计和合成一种能够进行序列特定合成的新型人造小分子机器.
  • 为了证明这个人工机器在产生的运行和效率.

主要方法:

  • 基于罗他森的分子机器的设计和合成.
  • 使用硫酸盐组进行代的氨基酸去除和转移.
  • 采用原生化学结合来形成键.
  • 展示了大约10^18个分子机器的并行操作.

主要成果:

  • 使用人工分子机器成功合成具有定义序列的.
  • 通过双重质谱测量来确认合成的序列.
  • 产生目标的毫克数量.

结论:

  • 开发的人工分子机器有效地以特定序列的方式模仿生物蛋白质合成.
  • 这项工作提出了人工分子机器用于合成的新方法.
  • 许多机器的并行运行证明了实际应用的可扩展性.