通过向前运输和被困在神经元生长体中的膜抗原的度
M P Sheetz1, N L Baumrind, D B Wayne
1Department of Cell Biology and Physiology, Washington University Medical School, St. Louis, Missouri 63110.
Cell
|April 20, 1990
概括
神经元生长通过将细胞表面分子移动到它们的前沿来导航神经系统. 这种活跃的运输和捕获机制涉及细胞骨元素,用于指导神经元生长.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 神经元生长指导神经系统的形成,通过在它们的前沿感知信号.
- 了解生长的分子动力学对于神经发育至关重要.
研究的目的:
- 研究细胞表面分子在神经元生长上的分布和运动.
- 阐明在生长头前边的分子运输和定位的基础机制.
主要方法:
- 使用抗体涂层黄金颗粒和视频增强差异干扰对比显微镜.
- 在培养的皮层神经元生长上观察神经细胞粘附分子 (N-CAM) 和2A1抗原的运动.
主要成果:
- 观察到黄金颗粒 (1-2微米/秒) 向前运输到生长的前边,在那里它们被困住.
- 在细胞素D治疗或ATP耗尽后,边缘的分子度仍然存在,但活跃运动已停止.
- 在filopodia中记录了细胞质聚合物的新型向外运动 (1.8微米/秒).
结论:
- 表明细胞表面分子的活跃向前运输和捕获涉及可逆性附着于细胞骨元素.
- 突出了细胞骨相互作用在指导神经元生长和路径发现中的作用.
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