一种结构类似于TFIIIA的指指蛋白,它只与Xenopusus中的5SRNA结合
K E Joho1, M K Darby, E T Crawford
1Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210.
Cell
|April 20, 1990
概括
仙人掌卵细胞含有5SRNA结合蛋白 (p43),与TFIIIA具有结构相似之处. 与TFIIIA不同,p43只结合5SRNA,而不是它的基因.
科学领域:
- 分子生物学分子生物学
- 发育生物学 发展生物学
- 在Xenopus laevis的研究中.
背景情况:
- 一种5SRNA结合蛋白,p43,在Xenopus卵细胞中丰富.
- p43是42S核糖蛋白储存粒子的一个组成部分.
研究的目的:
- 从Xenopus laevis和Xenopus borealis中克隆和测序p43cDNA.
- 为了比较p43与TFIIIA,另一个5SRNA结合蛋白.
主要方法:
- 来自Xenopus的p43和TFIIIA的cDNA克隆和测序.
- 氨基酸序列分析和同质性比较.
主要成果:
- p43和TFIIIA的结构相似,包括九个指.
- p43中的七根指与TFIIIA中的七根指的尺寸完全相同.
- 氨基酸同质性集中在保存的指残留物中.
- p43只与5SRNA结合,而TFIIIA与5SRNA及其基因结合.
结论:
- p43是一种独特的5SRNA结合蛋白,具有特定的RNA结合功能.
- 结构上的相似性表明p43和TFIIIA之间有共同的进化起源或功能关系.
相关概念视频
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