人类TFIID在一个重组的结构状态中与核心促销者DNA结合
Michael A Cianfrocco1, George A Kassavetis, Patricia Grob
1Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA.
Cell
|January 22, 2013
概括
人类TFIID,一个关键的转录因子,存在于两个不同的结构状态. 结合促进体DNA的TFIIA稳定了一种对转录启动和DNA识别至关重要的状态.
科学领域:
- 分子生物学分子生物学
- 结构生物学是结构生物学.
- 生物化学 生物化学
背景情况:
- 了解甲基动物转录对于破译细胞决策过程至关重要.
- 对转录启动,特别是DNA识别的结构性见解至关重要.
研究的目的:
- 在促进者DNA识别过程中阐明人类TFIID的结构机制.
- 调查TFIIA在调节TFIID结构和功能的作用.
主要方法:
- 单粒子电子显微镜 (EM) 用于可视化TFIID促进器DNA复合体.
- 用DNA标记和足迹技术来绘制促销元件的地图.
- 低温电子显微镜 (cryo-EM) 的研究.
主要成果:
- 人类TFIID在两个不同的结构状态中被观察到,其不同之处在于其叶片A的显著转移.
- 结合促进体DNA的TFIIA诱导TFIID的形状变化,促进促进体的识别.
- 在TFIID-TFIIA-DNA复合体内映射了特定的促进元件 (TATA,Inr,MTE,DPE).
结论:
- 两个TFIID符合者的存在表明了不同的功能角色和潜在的监管目标.
- TFIIA作为一个关键的调节器,稳定转录启动所需的TFIID的促进器结合状态.
相关概念视频
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:40The Eukaryotic Promoter Region
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
02:40The Eukaryotic Promoter Region
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...