潜伏增强剂通过在差异化细胞中的刺激来激活
Renato Ostuni1, Viviana Piccolo, Iros Barozzi
1Department of Experimental Oncology, European Institute of Oncology, Via Adamello 16, 20139 Milan, Italy. renato.ostuni@ieo.eu
Cell
|January 22, 2013
概括
这项研究揭示了潜在的增强剂在刺激时会变得活跃,形成表观遗传记忆. 这些未披露的增强剂持续存在,增强未来对环境因素的反应.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 免疫学 免疫学 免疫学
背景情况:
- 终端分化细胞通常具有由谱系特异转录因子 (TFs) 维护的固定的增强剂集.
- 刺激激活的TF通常与这种已建立的增强剂列表结合.
研究的目的:
- 确定和描述一种新的增强剂类别,称为"潜增强剂".
- 研究增强剂在响应细胞刺激时的动态调节及其在表观遗传记忆中的作用.
主要方法:
- 在终端分化的巨细胞中对TF结合和基因组修饰的全基因组分析.
- 刺激巨细胞和评估增强剂活性和TF结合的变化.
- 对增强剂在再刺激后的持久性和功能的分析.
主要成果:
- 潜在增强剂被确定为基因组区域缺乏TF结合和增强剂标记在未刺激的分化细胞中.
- 巨细胞刺激导致了连续的TF结合和在潜在的增强剂中获得增强剂标记.
- 在刺激停止后,暴露的增强剂的一个子集仍然存在,在重新刺激时调解增强的反应.
- 这表明,Cis-regulatory剧目的刺激特异性扩展.
结论:
- 潜伏增强剂代表了cis调节格局的动态组成部分,对细胞外刺激做出反应.
- 暴露的增强剂的持久性有助于形成表观遗传记忆,为未来与环境因素相遇的细胞做准备.
- 这种机制扩大了功能增强剂的范围,并增强了细胞的响应能力.
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