勇敢的心,一个长的非编码RNA,需要进行心血管血统承诺.
Carla A Klattenhoff1, Johanna C Scheuermann, Lauren E Surface
1Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Cell
|January 29, 2013
概括
勇敢的心脏 (Bvht),一种新的长非编码RNA,对于建立心血管血统至关重要. 这种与心脏相关的lncRNA指导胚胎干细胞向心脏命运分化,并调节关键的发育基因.
科学领域:
- 发育生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 长非编码RNAs (lncRNAs) 在发育中发挥作用,但它们在血统承诺中的功能基本上是未知的.
- 了解心血管发育的分子机制对于再生医学至关重要.
研究的目的:
- 识别和描述涉及心血管血统承诺的新型 lncRNA.
- 阐明心脏相关 lncRNA Braveheart (Bvht) 在哺乳动物心脏发育中的功能.
主要方法:
- 胚胎干细胞 (ESC) 分化试验.
- 对基因表达的分析 (qRT-PCR,RNA-seq).
- 染色体免疫沉 (ChIP) 测试用于评估表观遗传修饰.
- 与SUZ12和MesP1.1的相互作用研究.
主要成果:
- Bvht对于中皮体分化为心脏血统至关重要.
- Bvht 调节心血管核心基因网络,并且在 MesP1.1 的上游作用.
- Bvht与PRC2的组成部分SUZ12相互作用,这表明它在表观遗传调节中的作用.
- 此外,Bvht还参与新生儿心肌细胞中维持心脏命运.
结论:
- 勇敢的心 (Bvht) 是一个关键的长非编码RNA,用于哺乳动物发育过程中建立心血管血统.
- 它通过涉及PRC2的表观遗传机制来调节心脏承诺.
- 这项研究强调了 lncRNAs 在发育过程中的重要性,并为心脏研究提供了一个新的目标.
相关概念视频
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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02:39lncRNA - Long Non-coding RNAs
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02:35RACE - Rapid Amplification of cDNA Ends
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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