细胞透多二硫化物的基质启动合成
Eun-Kyoung Bang1, Giulio Gasparini1, Guillaume Molinard1
1School of Chemistry and Biochemistry, University of Geneva, Geneva, Switzerland.
Journal of the American Chemical Society
|February 1, 2013
概括
研究人员开发了一种新方法,可以直接在表面上制造穿透细胞的聚硫化物. 这一突破使得有针对性的药物输送和通过在细胞吸收后释放本地基质来降低毒性.
科学领域:
- 聚合物化学 聚合物化学
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
背景情况:
- 表面启动的聚合提供了对聚合物生长的控制.
- 穿透细胞的聚合物对于细胞内药物输送至关重要.
- 尽量减少输送系统的毒性是一个关键的挑战.
研究的目的:
- 开发一种方法,在各种基板上培养细胞透的聚硫化物.
- 研究这些聚合物的基质启动的聚合和随后的脱聚合.
- 评估聚合和脱聚合对细胞运输活动的影响.
主要方法:
- 基质启动的聚合利用硫化基质和扩散剂与应变的二硫化物 (脂酸) 和瓜尼尼离子.
- 在室温和中性pH值下快速聚合 (<5分钟).
- 使用迪三醇诱导的脱聚合,化囊泡,凝透色谱和光共振能量转移进行表征.
主要成果:
- 在选择的基板上直接成功生长细胞透的聚硫化物.
- 证明了基质启动的聚合和还原性脱聚合.
- 在化囊泡中观察到与聚合和脱聚合相关的可逆运输活性.
结论:
- 表面启动的聚合提供了一条通往细胞透的多二硫化的多功能途径.
- 开发的系统可以控制基质的释放,并最大限度地降低毒性.
- 这种方法对先进的细胞内输送系统具有前景.
相关概念视频
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