血液传递的昼夜信号刺激了行为动态和SRF活动中的每日振荡
Alan Gerber1, Cyril Esnault, Gregory Aubert
1Department of Molecular Biology, Sciences III, University of Geneva, and National Centre of Competence in Research Frontiers in Genetics, 1211 Geneva, Switzerland.
Cell
|February 5, 2013
概括
研究人员将血清响应因子 (SRF) 确定为直接早期转录因子 (IETF),响应血液传递的信号. 这一发现解释了外围组织如何与身体同步基因表达.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 周围组织的基因表达受局部振荡器或来自大脑主钟的系统性线索的调节.
- 系统信号可以触发即时的早期转录因子 (IETF),以控制节奏转录.
研究的目的:
- 为了识别由白天血液传播信号诱导的IETF.
- 了解将系统线索与外围昼夜基因表达联系在一起的机制.
主要方法:
- 开发了合成Tandem重复PROMoter (STAR-PROM) 选,这是识别IETF的一种公正的方法.
- 利用STAR-PROM分析随机DNA序列中的转录因子结合位.
- 调查了IETFs在对振荡血清蛋白质的反应中的作用.
主要成果:
- 鉴定了血清反应因子 (SRF) 作为IETF对人类和动物血清中振荡信号蛋白反应的反应因子.
- 已经证明,小鼠肝脏中的SRF受到白天动力学动态变化的调节.
- 在小鼠肝脏中显示了SRF联合激活剂肌肉糖相关转录因子-B (MRTF-B) 的节奏核转位.
结论:
- 在外围组织中,SRF充当全身昼夜信号的关键调解者.
- 动因动态和MRTF-B核转位代表了SRF昼间调节的新机制.
- 这可以了解周围的昼夜节律是如何与中央时钟同步的.
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