Ubiad1是一种抗氧化酶,通过合成CoQ10调节eNOS活性
Vera Mugoni1, Ruben Postel, Valeria Catanzaro
1Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, 10126 Torino, Italy.
Cell
|February 5, 2013
概括
斑马鱼barolo (bar) 突变体显示,UBIAD1,一种辅酶Q10 (CoQ10) 合成剂,可以保护心血管健康. 丢失UBIAD1会导致氧化应激和心力衰竭,突出其在抗氧化防御和NO信号传递中的作用.
科学领域:
- 心血管生物学 心血管生物学
- 氧化压力研究研究 氧化压力研究
- 分子遗传学 分子遗传学
背景情况:
- 抗氧化剂网络对心血管健康至关重要,它可以保护我们免受活性氧物种 (ROS) 的侵害.
- 在斑马鱼中被识别为barolo (bar) 的基因ubiad1,表现出显著的抗氧化特性.
研究的目的:
- 研究UBIAD1在心血管保护和氧化应激中的作用.
- 阐明UBIAD1影响心血管健康和NO信号的机制.
主要方法:
- 在斑马鱼中分析一个零等位基因 (barolo),以研究ubiad1功能.
- 研究辅酶Q10 (CoQ10) 合成及其对ROS介导损伤的影响.
- 评估UBIAD1,CoQ10和内皮氧化合成酶 (eNOS) 活性之间的相互作用.
主要成果:
- 酒吧突变体表现出与氧化应激和ROS诱导的细胞损伤有关的心血管衰竭.
- 人类UBIAD1,非线粒体前基转移酶,在戈尔吉细胞中合成CoQ10,其损失降低了细胞质CoQ10水平.
- 降低UBIAD1功能导致血管细胞中ROS介导的脂质过氧化.
- 抑制eNOS可以改善Ubiad1依赖的心血管氧化损伤,这表明它在NO信号传递中发挥了作用.
结论:
- UBIAD1被确定为一种关键的非线粒体CoQ10形成酶,对心血管保护至关重要.
- UBIAD1调节eNOS活动,在心血管抗氧化防御和NO信号通路中发挥关键作用.
相关概念视频
01:30Enzyme Inhibition
Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
01:29Role of Reduced Coenzymes NADH and FADH₂
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
01:43Electron Transport Chain: Complex III and IV
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
01:24Cofactors and Coenzymes
Enzymes are proteins made of amino acids. The functional group of each constituent amino acid catalyzes a wide variety of chemical reactions via ionic interactions or acid-base reactions. However, amino acids cannot catalyze oxidation-reduction and group transfer reactions and need to be aided by non-protein components called cofactors. Cofactors are also referred to as the chemical teeth of an enzyme.
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
01:27Cofactors and Coenzymes
Enzymes require additional components for proper function. There are two such classes of molecules: cofactors and coenzymes. Cofactors are metallic ions and coenzymes are non-protein organic molecules. Both of these types of helper molecule can be tightly bound to the enzyme or bound only when the substrate binds.
01:27Cofactors and Coenzymes
Enzymes require additional components for proper function. There are two such classes of molecules: cofactors and coenzymes. Cofactors are metallic ions and coenzymes are non-protein organic molecules. Both of these types of helper molecule can be tightly bound to the enzyme or bound only when the substrate binds.