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相关概念视频

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Allosteric Regulation01:08

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
Allosteric Regulation01:08

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
From DNA to Protein03:06

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

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相关实验视频

Updated: May 14, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

通过DNA测试全精子.

Sangjin Kim1, Erik Broströmer, Dong Xing

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Science (New York, N.Y.)
|February 16, 2013
PubMed
概括
此摘要是机器生成的。

研究人员发现了DNA异构,即附近的蛋白质改变了DNA与蛋白质的结合. 这种互动互动.

更多相关视频

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

相关实验视频

Last Updated: May 14, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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科学领域:

  • 分子生物学分子生物学
  • 生物物理学的生物物理.
  • 遗传学 是一个遗传学.

背景情况:

  • 通过遥远的结合事件调节蛋白质功能的Allostery对于蛋白质来说已经得到了很好的确立.
  • 对DNA-蛋白相互作用的化效应仍然不太了解,这代表着一个重要的知识差距.
  • 了解DNA-蛋白相互作用对于基因调节和细胞过程至关重要.

研究的目的:

  • 为了研究和描述DNA与蛋白质相互作用中的菌现象.
  • 为了确定DNA全osteric效应的物理基础和周期性.
  • 在生物系统中探索DNA异质的生理影响.

主要方法:

  • 在不同的蛋白质-蛋白质分离下,实验确定蛋白质-DNA结合亲和力.
  • 三元复合体自由能量作为蛋白质间距离的函数的分析.
  • 分子动力学模拟以阐明DNA全菌的结构基础.
  • 在体内对细菌进行研究,以评估对基因表达的影响.

主要成果:

  • 蛋白质与DNA的特定结合因附近蛋白质的存在而显著稳定或不稳定.
  • 三元复合体的自由能量以大约10个基对的周期性振荡 (B-DNA螺旋曲率).
  • 基因菌起源于DNA双螺旋结构的变形,由模拟支持.
  • 这种现象影响细菌中的基因表达,并影响核细胞附近的转录因子结合.

结论:

  • 基因化是一种新发现的调节DNA-蛋白相互作用的机制.
  • DNA的螺旋结构在调解这些全效应方面发挥着至关重要的作用.
  • 在生物体内,DNA全ostery在调节基因表达和蛋白质结合方面具有显著的生理相关性.