NeST长ncRNA控制微生物易感性和干扰素γ位的表观遗传激活
J Antonio Gomez1, Orly L Wapinski, Yul W Yang
1Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Cell
|February 19, 2013
概括
一种新的长非编码RNA,NeST,调节基因表达和免疫反应. NeST通过改变干扰素-马位点的表观遗传标记来控制对病毒和细菌病原体的敏感性.
科学领域:
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 长非编码RNAs (lncRNAs) 被认为是基因表达的关键调节者.
- 鼠类病毒易感点Tmevp3影响宿主对病原体的反应.
- 与B10.S小鼠相比,SJL/J小鼠表现出不同的免疫表型.
研究的目的:
- 为了确定Tmevp3位点内的因果基因.
- 阐明已识别的lncRNA在宿主防御中的功能.
- 了解NeST介导的基因调节的分子机制.
主要方法:
- 在SJL/J和B10.S小鼠之间进行遗传交叉,以确定Tmevp3位点.
- 对NeST lncRNA的转基因表达.
- 用RNA结合测试来识别蛋白质相互作用体.
- 染色体免疫沉以评估组织蛋白修饰.
主要成果:
- 类似增强剂的lncRNA NeST被确定为Tmevp3表型的因果因素.
- 仅仅转基因NeST表达就重新总结了所有与SJL/J位点相关的表型.
- 尼斯特RNA与WDR5结合,并在干扰素- (IFN-γ) 位点改变基因素甲基化.
- NeST调节IFN-γ的表达和对泰勒病毒和沙门氏菌的敏感性.
结论:
- NeST是免疫反应和病原体易感性的关键调节者.
- 这种lncRNA以表观遗传作用来控制IFN-γ的表达.
- NeST代表了传染病的新型治疗点.
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