相关实验视频
Updated: May 13, 2026

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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
循环RNA是一种大型的动物RNA类型,具有调节功效
Sebastian Memczak1, Marvin Jens, Antigoni Elefsinioti
1Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
Nature
|March 1, 2013
概括
循环RNAs (circRNAs) 是一种新发现的RNA分子类. 这些稳定的分子通过结合microRNAs (miRNAs) 作为关键的转录后调节剂,影响基因表达.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 发育生物学 发展生物学
背景情况:
- 循环RNAs (circRNAs) 代表了一个神秘的RNA分子类,其功能基本上是未知的.
- 它们的稳定性和潜在的监管作用需要进行系统的调查.
研究的目的:
- 在不同物种中系统地识别和分析循环RNA (circRNAs).
- 研究circRNAs的功能作用和调节潜力,特别是在转录后调节方面.
主要方法:
- 人类,小鼠和线虫样本的高通量RNA测序.
- 计算分析用于检测和描述circRNAs.
- 在斑马鱼中进行功能性测试,以评估circRNAs对发育的影响.
主要成果:
- 数以千计的稳定,表达良好的circRNAs被确定,许多表现出组织和发育阶段特定的表达模式.
- 发现一种特定的人类circRNA,CDR1as,可以结合众多的microRNA (miRNA) 效应复合体,特别是作为miR-7的强有力的海绵.
- CDR1as作为一个miRNA对手,在斑马鱼的实验证据表明其在中脑发育调节中的作用.
结论:
- 循环RNAs (circRNAs) 构成一个重要的后转录调节器类.
- 在circRNA形成中的表子的头到尾拼接表明了来自编码序列的新型调节潜力.
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Ribosomal RNA Synthesis
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Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

