相关实验视频
Updated: May 10, 2026

12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 27, 2013
化通过诱导致病的TH17细胞驱动自身免疫性疾病
Markus Kleinewietfeld1, Arndt Manzel, Jens Titze
1Departments of Neurology and Immunobiology, Yale School of Medicine, 15 York Street, New Haven, Connecticut 06520, USA. markus.kleinewietfeld@yale.edu
Nature
|March 8, 2013
概括
高盐摄入量可以促进致病性T辅助细胞17 (TH17),增加自身免疫性疾病的风险. 这项研究确定盐是影响TH17细胞发育和致病性的关键环境因素.
科学领域:
- 免疫学 免疫学 免疫学
- 环境健康 环境健康
背景情况:
- 自身免疫性疾病增加了,鉴定了遗传因素,但未知环境触发因素.
- 产生中白素-17的T辅助细胞17 (TH17) 在自身免疫性疾病中至关重要.
- 影响TH17细胞的环境因素在很大程度上仍未被描述.
研究的目的:
- 研究环境因素,特别是盐度对TH17细胞诱导和致病性的影响.
- 阐明盐影响TH17细胞发育的分子机制.
主要方法:
- 在不同盐 (NaCl) 度下,小鼠和人类TH17细胞的两极分化.
- 对信号通路的分析,包括p38/MAPK,NFAT5和SGK1.1.
- 评估TH17细胞表型和细胞因子的产生 (GM-CSF,TNF-α,IL-2).
- 在高盐饮食的小鼠中评估实验性自身免疫脑膜炎 (EAE) 严重程度.
主要成果:
- 高盐度显著增强了和人类TH17细胞的诱导.
- 盐诱导的TH17细胞发育是由p38/MAPK通路介导的,涉及NFAT5和SGK1.
- 高盐生成的TH17细胞表现出增加的致病性,稳定性和促炎性细胞因子的产生.
- 高盐饮食加剧了EAE的严重程度,与TH17细胞透率的增加相关.
结论:
- 增加盐的摄入量被认为是自身免疫性疾病的潜在环境风险因素.
- 盐促进病原性TH17细胞的发展,有助于疾病的严重程度.
- 针对盐诱导的途径可能为自身免疫性疾病提供新的治疗策略.
相关概念视频
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Autoimmune Disorders
Autoimmune diseases are a group of disorders in which the body's immune system mistakenly attacks its own cells, tissues, and organs. This results from an overactive immune response against substances and tissues normally present in the body. Let's delve into the concept and mechanism of autoimmune diseases from an immune system point of view, explore different causes and examples of such diseases, and discuss potential solutions.
Concept and Mechanism of Autoimmune Diseases
The immune system...
Concept and Mechanism of Autoimmune Diseases
The immune system...
Type I Diabetes II: Pathophysiology
Type 1 diabetes mellitus arises from an immune-mediated destruction of pancreatic β-cells, resulting in an absolute deficiency of insulin. This process develops in genetically susceptible individuals when autoimmunity, environmental exposures, and immunologic dysregulation converge to trigger a targeted attack on the insulin-producing cells of the pancreas. The β-cells are located within the islets of Langerhans and are essential for regulating blood glucose by facilitating cellular uptake of...
Inflammatory Bowel Disease III: Crohn's Disease
Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...

