滴基酶4是新兴的人类冠状病毒-EMC的功能性受体
V Stalin Raj1, Huihui Mou, Saskia L Smits
1Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
Nature
|March 15, 2013
概括
研究人员确定了二乙酶4 (DPP4) 作为人类冠状病毒-伊拉斯木斯医疗中心 (hCoV-EMC) 的细胞受体. 这一发现提供了对病毒的洞察力.
科学领域:
- 病毒学 病毒学
- 传染性疾病 传染性疾病
- 分子生物学分子生物学
背景情况:
- 人类冠状病毒通常会导致轻度呼吸道疾病.
- 像SARS和hCoV-EMC这样的新兴冠状病毒可以导致严重的,致命的下呼吸道感染.
- hCoV-EMC与蝙蝠冠状病毒具有遗传相似性.
研究的目的:
- 为了确定hCoV-EMC.的细胞受体.
- 了解hCoV-EMC.的宿主范围和病原性.
- 为制定干预策略提供信息.
主要方法:
- 与hCoV-EMC尖端蛋白S1域共同净化DPP4.
- 使用抗DPP4抗体抑制hCoV-EMC感染.
- 在非敏感细胞中表达人类和蝙蝠DPP4,以测试传染性.
主要成果:
- 双基化酶4 (DPP4) 被确定为hCoV-EMC的功能受体.
- DPP4特别与hCoV-EMC尖端蛋白的S1域结合.
- 反DPP4抗体在细胞培养中阻止了hCoV-EMC感染.
- 人类和蝙蝠DPP4使hCoV-EMC感染在非宽容细胞中成为可能.
结论:
- DPP4是hCoV-EMC细胞进入和宿主热带的关键决定因素.
- 跨物种的DPP4的保存性表明hCoV-EMC的广泛宿主范围的潜力.
- 了解DPP4-hCoV-EMC相互作用对于开发抗病毒疗法和预测疾病传播至关重要.
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