USP33通过对中心蛋白 CP110 的二基化来调节中心体生物发生
Ji Li1, Vincenzo D'Angiolella, E Scott Seeley
1Department of Pathology and Cancer Institute, Smilow Research Center, New York University School of Medicine, 522 1st Avenue, New York, New York 10016, USA.
Nature
|March 15, 2013
概括
USP33,一种二基化酶,通过控制CP110水平来调节中心体重复. 这一发现揭示了维持细胞分裂精度的新机制,并提供了潜在的癌症治疗策略.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 中心细胞复制对于细胞分裂至关重要;错误会导致基因组不稳定.
- CP110调节中心的重复和延长,其水平由SCF ((环素F) 无化密切控制.
- CP110的调节失调导致中心细胞放大或中心细胞组合和纤维生成的缺陷.
研究的目的:
- 为了确定中心体重复的新型调节者.
- 阐明duebiquitinating酶在控制CP110水平和中心体恒温中的作用.
- 探索治疗策略,以向瘤发生中的中心细胞放大.
主要方法:
- 研究了USP33与CP110在人体细胞中的相互作用和局部化.
- 评估了USP33在CP110.0上的脱化活性.
- 分析了USP33剥离对中枢细胞体放大和细胞分裂的影响.
主要成果:
- 在S和G2/M阶段,USP33与CP110相互作用,并局部化为中心离子.
- USP33 特别是二维基因酸 CP110,抵消SCF ((环素F) 介导的泛基因化.
- USP33促进了超数的中心焦点,而它的缺失破坏了CP110的稳定,抑制了中心放大.
结论:
- USP33是第一个已识别的中心离子二基化酶,它调节了中心体恒温.
- USP33计数SCF ((环素F) 介导的CP110降解,保持中心细胞复制控制.
- 这些发现表明USP33是具有中心细胞放大功能的癌症的潜在治疗标.
相关概念视频
02:25Centrosome Duplication
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02:25Centrosome Duplication
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02:19The Spindle Assembly Checkpoint
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00:50Anaphase Promoting Complex
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00:50Anaphase Promoting Complex
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