相关实验视频
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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
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基因组架构:跨相染色体的域组织
Wendy A Bickmore1, Bas van Steensel
1MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH42XU, UK. wendy.bickmore@igmm.ed.ac.uk
Cell
|March 19, 2013
概括
染色体结构对于基因调节和基因组稳定至关重要,涉及不同的领域和非随机的空间安排. 先进的绘图和建模揭示了整合的线性和3D染色体组织.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 染色体间相架构对于基因表达和基因组维护至关重要.
- 染色体具有线性细分到具有独特蛋白质组成的域.
- 染色体的空间组织不是随机的,具有局部和远程接触.
研究的目的:
- 探索染色体结构和基因调节之间的关系.
- 整合染色体组织的线性和三维 (3D) 视图.
- 通过染色体结构洞察了解基因组维护.
主要方法:
- 利用各种全基因组映射技术进行高分辨率绘图.
- 使用显微镜可视化染色体结构.
- 应用计算建模来解释空间组织数据.
主要成果:
- 染色体域组成和空间联系的详细图表.
- 高分辨率测绘揭示了复杂的局部和远程染色体相互作用.
- 线性和3D数据的整合提供了染色体组织的连贯视图.
结论:
- 染色体结构显著影响基因调节和基因组稳定性.
- 结合地图,显微镜和建模,提供了对核组织的全面了解.
- 这种综合方法有助于深入了解染色体结构的功能作用.
相关概念视频
02:40Chromosome Structure
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
02:40Chromosome Structure
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
01:17Karyotyping
Overview
01:17Karyotyping
Overview
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
01:32Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...