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重编程诱导多能性的表观遗传学
Bernadett Papp1, Kathrin Plath
1Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Cell
|March 19, 2013
概括
将细胞重新编程成诱导多能干细胞 (iPSC) 涉及逐步的变化. 早期的表观遗传原始化对于以后的多能性至关重要,但人类iPSC中的X染色体不稳定性需要注意.
科学领域:
- 干细胞生物学 干细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 细胞重新编程的细胞重编程.
背景情况:
- 诱导多能干细胞 (iPSC) 是通过细胞重编程生成的.
- 重编程是一个逐步的过程,涉及转录和染色质状态的变化.
- 表观遗传修饰在重编程的效率和结果中起着至关重要的作用.
研究的目的:
- 阐明细胞重新编程成iPSC的逐步机制.
- 突出早期表观遗传事件在多能诱导中的重要性.
- 讨论重编程因素和细胞外条件对这个过程的影响.
主要方法:
- 重编程因子结合和转录动态的表征.
- 在重编程过程中分析染色质状态的变化.
- 对表观遗传原始事件的调查.
主要成果:
- 重编程因子的结合,转录和染色质的修饰发生在顺序上.
- 早期的表观遗传原始事件对于成功的多能诱导至关重要.
- 重编程因子水平,固体测量和细胞外条件显著影响重编程结果.
- 人类iPSC中X染色体的表观遗传不稳定性已被确定.
结论:
- 了解重编程的逐步性质,包括表观遗传动力学,是改善iPSC应用的关键.
- 新型重编程因子尾酒的理性设计是最近的进展所实现的.
- 在人类iPSC中发现的X染色体的表观遗传不稳定性需要仔细考虑未来的研究和治疗用途.
相关概念视频
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