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相关概念视频

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

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相关实验视频

Updated: May 13, 2026

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

核定位是指核定位.

Gregg G Gundersen1, Howard J Worman

  • 1Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA. ggg1@columbia.edu

Cell
|March 19, 2013
PubMed
概括
此摘要是机器生成的。

细胞核,通常是中央的,以不对称的方式移动,以支持细胞功能,如分裂和迁移. 蛋白质和细胞骨力量驱动这种运动,对健康和预防疾病至关重要.

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Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion
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Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

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Heterokaryon Technique for Analysis of Cell Type-specific Localization
09:31

Heterokaryon Technique for Analysis of Cell Type-specific Localization

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相关实验视频

Last Updated: May 13, 2026

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion
09:03

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

Published on: April 13, 2019

Heterokaryon Technique for Analysis of Cell Type-specific Localization
09:31

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Published on: March 11, 2011

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 细胞核通常是中心的,但在关键的细胞过程中会改变位置.
  • 核定位对于细胞功能,组织和信号传递至关重要.

研究的目的:

  • 探索参与核运动的蛋白质机械和细胞骨力量.
  • 为了突出破坏核定位在疾病中的影响.
  • 讨论核位置在细胞组织和信号传递中的作用.

主要方法:

  • 关于核力学和蛋白质相互作用的文献综述.
  • 对细胞骨动力学和力量生成的分析.
  • 检查与核定位缺陷相关的疾病模型.

主要成果:

  • 确定了关键蛋白质"工具箱",它们调解了核运动和定.
  • 证明了细胞骨力量与核运输的合.
  • 将异常的核定位与各种遗传疾病联系在一起.

结论:

  • 核定位是一个由特定的蛋白质复合体和细胞力量调节的动态过程.
  • 适当的核定位对于正常的细胞功能和生物体健康至关重要.
  • 对核定位的进一步研究可能会揭示相关疾病的新治疗点.