使用基于电子磁共振的技术,用于在体内组织中同时监测氧气和pH值的酸探针
Ilirian Dhimitruka1, Andrey A Bobko, Timothy D Eubank
1Dorothy M. Davis Heart & Lung Research Institute and Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Internal Medicine, The Ohio State University, Columbus, Ohio 43210, USA.
Journal of the American Chemical Society
|March 23, 2013
概括
一个新的双重功能探头,p1TAM-D,可以同时测量组织中的pH和氧气水平. 这种偏磁探头为体内监测提供了高灵敏度和精度,有助于癌症研究.
科学领域:
- 参磁探测器的探测器
- 电子偏磁共振 (EPR) 光谱学
- 生物医学传感传感器
背景情况:
- 持久的三甲基基 (TAMs) 之前被概念化为双功能的pH和氧探针.
- 将可电离组纳入TAM结构是开发响应式探测器的关键.
研究的目的:
- 为了合成和描述用于双功能EPR应用的不对称的单酸TAM探针.
- 为了评估探针的灵敏度和准确性,同时进行体内pH和氧气监测.
主要方法:
- 一个化单化TAM衍生物 (p1TAM-D) 的合成.
- 描述EPR光谱特性,包括线宽和对氧和pH的敏感性.
- 在乳腺瘤携带小鼠的体内L频段EPR研究.
主要成果:
- 合成的p1TAM-D具有非常窄的线宽,导致对pO2的高灵敏度 (精度约为1 mmHg).
- 素组在生理范围 (pH 5.98.2) 内赋予了高精度 (±0.05) 的pH灵敏度.
- 在瘤和正常组织之间观察到细胞外pH和pO2的明显差异.
结论:
- p1TAM-D 探针对 pH 和氧气都表现出独立的灵敏度,从而实现了双重功能.
- L频段EPR研究证实了该探针在体内同时监测组织氧气和pH值的实用性.
- 这种化TAM探测器代表了先进的生物医学传感和癌症研究的独特工具.
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