一个由Ro自身抗原和非编码RNA雕塑的RNA降解机器
Xinguo Chen1, David W Taylor, Casey C Fowler
1Department of Cell Biology, Yale School of Medicine, New Haven, CT 06510, USA.
Cell
|April 2, 2013
概括
细菌使用一个YRNA架构的机器,涉及Ro ortholog Rsr和PNPase,来降解RNA. 这种核糖核蛋白复合体改变了结构化RNA分解的酶特异性.
科学领域:
- 细菌学 细菌学是一门学科.
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 许多细菌拥有一个与YRNAs结合的Ro自身抗原原体.
- 在Deinococcus radiodurans中,Ro ortholog Rsr参与了rRNA处理和衰变.
- 对于Rsr和YRNA功能的确切机制仍然不清楚.
研究的目的:
- 在细菌RNA处理中阐明Ro ortholog Rsr和YRNA的机制.
- 描述由Rsr,YRNA和PNPase组成的核糖蛋白复合体.
- 研究ncRNA在改变酶基质特异性的作用.
主要方法:
- 单粒子电子显微镜用于确定复杂结构.
- 原子模型对接到电子显微镜重建.
- 生物化学测试以评估RNA降解活性.
主要成果:
- Rsr和多核酸酶 (PNPase) 构成一个由YRNA架构的RNA降解机器.
- Rsr促进了单链RNA向PNPase活性部位的道化.
- Rsr和YRNA使PNPase能够有效地降解结构RNA.
- 在沙门氏菌Typhimurium中观察到一个类似的复合体,涉及Ro ortholog,ncRNA和PNPase.
结论:
- 确定了一种用于RNA降解的新型细菌核糖蛋白机器.
- 证明ncRNA可以通过绑定蛋白质辅因子来修改酶基质的特异性.
- 揭示了跨细菌物种RNA处理和降解的保存机制.
相关概念视频
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...