生理细胞外化物谷二硫酸在表面功能化合体氨酸颗粒上的吸附和定向
Fabian Meder1, Henrik Hintz, Yvonne Koehler
1Faculty of Production Engineering, Advanced Ceramics, University of Bremen, Bremen, Germany.
Journal of the American Chemical Society
|April 10, 2013
概括
颗粒的表面化学决定了生物分子吸附. 在体上调整氨基酸或硫酸盐等表面组,影响二硫酸 (GSSG) 结合和纳米医学应用的方向.
科学领域:
- 合体和表面科学科学
- 生物材料工程 生物材料工程
- 纳米技术 纳米技术
背景情况:
- 设计用于生物技术和纳米医学应用的材料需要了解生物分子对体颗粒的吸附.
- 表面化学在控制这些相互作用方面发挥着至关重要的作用.
研究的目的:
- 研究合体颗粒的不同表面化学成分如何影响谷二硫化物 (GSSG) 的吸附和定向.
- 阐明粒子表面功能化,泽塔潜力和GSSG吸附行为之间的关系.
主要方法:
- 用氨基,碳酸盐,酸盐和硫酸盐组对合体颗粒 (180 nm) 的功能化.
- 在改性颗粒上进行GSSG的吸附研究.
- 吸附动力学,等温 (Langmuir) 和可逆性的分析.
- 通过谷氨释放和泽塔电位测量评估GSSG方向.
主要成果:
- GSSG在原生,氨基和硫酸盐功能化的上被吸附,但在碳酸盐或酸盐功能化的颗粒上没有.
- 吸附是快速的 (≤5分钟),与盐可逆,并遵循兰格穆尔动力学.
- GSSG的导向有所不同:通过碳酸盐组与原生/氨基结合,以及通过氨基组与硫酸结合.
结论:
- 调整合体颗粒的表面化学成分,可以控制GSSG的吸附和导向.
- 这为设计具有特定生物分子相互作用配置文件的材料提供了一条途径,用于纳米医学应用.
相关概念视频
01:22Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation
Glutathione, a tripeptide made up of glutamate, cysteine, and glycine, is a critical player in the detoxification of drugs and xenobiotics via a process known as glutathione conjugation or mercapturic acid formation. This phase II biotransformation reaction involves the covalent binding of glutathione to a drug or its metabolite, enhancing the compound's water solubility and enabling its excretion.
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
01:09Colloidal precipitates
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...