高水平的半合成生产强大的抗疟疾药物artemisinin
C J Paddon1, P J Westfall, D J Pitera
1Amyris, Inc., 5885 Hollis Street, Suite 100, Emeryville, California 94608, USA. paddon@amyris.com
Nature
|April 12, 2013
概括
科学家们利用酵母技术制造出艺术氨酸,这是抗疟疾药物艺术氨酸的前体. 这种合成生物学方法确保了稳定,负担得起的素供应,这对于全球抗击疟疾至关重要.
科学领域:
- 合成生物学 合成生物学
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
背景情况:
- 疟疾仍然是一个重大的全球卫生负担,每年有数百万病例和数十万死亡.
- 建议使用基于美素的组合疗法 (ACT) 治疗疟疾,但依赖于美素,一种具有不稳定的供应链的化合物.
- 来自植物的素生产面临着价格波动和短缺的挑战,影响了ACT制造业.
研究的目的:
- 开发一种稳定,负担得起的阿尔特美西宁来源,用于治疗疟疾.
- 改造Saccharomyces cerevisiae (面包酵母) 以产生高产的艺术氨酸,一种关键的艺术氨酸前体.
- 建立一个可扩展和高效的过程,用于将艺术氨酸转化为艺术氨酸.
主要方法:
- 利用合成生物学来设计酵母菌株用于艺术氨酸生物合成.
- 鉴定并结合了一种新的植物脱酶和第二个细胞染色体,以优化生物合成途径.
- 开发了一种可扩展的化学工艺,使用单片氧来从酸中合成素.
主要成果:
- 实现了25克/升的酸发酵量,这与之前的尝试相比是显著的改善.
- 在没有专门的设备的情况下,建立了一种实用和高效的化学转化,将艺术氨酸转化为艺术氨酸.
- 创建了一个可行的工业工艺,用于半合成的阿尔特梅西宁生产.
结论:
- 开发的酵母菌株和化学工艺为半合成美素生产提供了稳定且负担得起的工业方法.
- 这项技术有可能降低抗疟疾治疗的成本,并改善发展中国家的治疗机会.
- 免费提供知识产权旨在最大限度地影响全球疟疾治疗的可获得性.
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