主转录因子和媒介在关键细胞身份基因中建立了超级增强剂
Warren A Whyte1, David A Orlando, Denes Hnisz
1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
Cell
|April 16, 2013
概括
主转录因子在胚胎干细胞 (ESC) 中形成超强增强剂,以控制多能性. 这些超级增强剂对于在多能细胞和分化细胞中保持细胞身份至关重要.
科学领域:
- * 发育生物学 发育生物学
- * 分子生物学 * 分子生物学
- * * 干细胞生物学
背景情况:
- * 主转录因子Oct4,Sox2和Nanog调节多能胚胎干细胞 (ESC) 的基因表达.
- * 这些因素结合增强剂元素,并招募调解者来激活基因表达程序.
- *在经济社会委员会中,这些监管要素的精确组织尚未完全理解.
研究的目的:
- *研究ESC中由主转录因子调节的增强器域的形成和功能.
- *为了描述这些新的增强器领域,称为超级增强器.
- * 了解超级增强剂在发育过程中维持细胞身份中的作用.
主要方法:
- *分析转录因子和调解器占用在ESC的增强元件.
- *超强增强特性的表征,包括大小,密度和转录活性.
- *涉及Oct4或Mediator降低水平的乱研究,以评估基因表达变化.
主要成果:
- * 主转录因子和中介因子在控制ESC多能性的基因上形成广泛的增强器域,称为超级增强器.
- * 超级增强剂在尺寸,转录因子密度和转录功效方面与典型增强剂不同.
- *降低Oct4或Mediator水平优先影响与超级增强剂相关的基因表达.
- * 超级增强剂也在分化细胞中被发现,与细胞类型特定的主转录因子和定义细胞身份的基因有关.
结论:
- * 超级增强剂是由主转录因子和ESC中调解者形成的关键调节枢纽.
- *这些领域在建立和维持多权国家方面发挥着关键作用.
- *超强增强剂是保护的调控结构,对于定义不同细胞类型的细胞身份至关重要.
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