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Nr4a1-依赖的Ly6C ((低) 单细胞监测内皮细胞并协调它们的处置

Leo M Carlin¹, Efstathios G Stamatiades, Cedric Auffray

¹Centre for Molecular and Cellular Biology of Inflammation, King's College London, London SE1 1UL, UK.

|April 16, 2013

概括

Ly6C ((低) 单细胞充当血管内管家. 它们在响应危险信号时通过中性粒细胞协调内皮细胞死亡,然后清除细胞残骸.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
血管生物学血管生物学

背景情况:

在血管平衡中依赖Nr4a1的Ly6C单细胞在血管平衡中的确切作用尚未完全理解.
这些单细胞通常在毛细血管中发现,在那里他们收集微粒.

研究的目的:

阐明Ly6C(低) 单细胞在对炎症刺激的反应中的功能.
研究这些单细胞与内皮细胞和中性粒细胞相互作用的机制.

主要方法:

使用了基因修饰的小鼠模型 (Nr4a1(-/-),Itgal(-/-),Tlr7(主机-/-BM+/+),Cx3cr1(-/-)).
给药的TLR7依赖性危险信号会扰乱的平衡.
采用中性粒细胞枯竭和基因操纵来阻止中性粒细胞的招募.

主要成果:

在TLR7激活时,Ly6C ((低) 单细胞被静脉内保留,招募中性粒细胞.
这种相互作用导致焦点内皮细胞死亡,随后由单细胞清除碎片.
单细胞发育,保留或中性粒细胞招募的遗传或药理学阻断废除了内皮细胞死亡.

结论:

Ly6C ((低) 单细胞作为血管内管家的功能,启动中性粒细胞介导的内皮细胞死亡对危险信号的反应.
这一过程受到TLR7信号的严格调节,并涉及单细胞随后的碎片细胞化.

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