基因组向原理是MBD蛋白家族的基因组向原理依赖于甲基化和独立于甲基化
Tuncay Baubec1, Robert Ivánek, Florian Lienert
1Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland.
Cell
|April 16, 2013
概括
研究人员绘制了甲基-CpG结合域 (MBD) 蛋白质的地图,揭示了它们根据甲基化密度如何与DNA结合. 这阐明了DNA甲基化读出机制和MBD蛋白的功能.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 基因甲基化是调节基因表达的关键表观遗传机制.
- 甲基-CpG结合域 (MBD) 蛋白质是DNA甲基化的关键读者.
- 了解MBD蛋白结合对于破译基因调节至关重要.
研究的目的:
- 系统地描述MBD蛋白质的全基因组分布.
- 创建MBD家族蛋白质的基因组图,包括突变体和异构体.
- 阐明MBD蛋白与甲基化和非甲基化DNA结合的机制.
主要方法:
- 开发了一种全基因组对色素相互作用因子的分析策略.
- 生成MBD蛋白家族成员的基因组图.
- 分析了与DNA甲基化密度相关的体内结合模式.
主要成果:
- MBD蛋白结合在很大程度上是局部DNA甲基化密度的线性函数.
- 主要结合需要功能MBD域和甲基-CpG.
- MBD蛋白质向甲基化,CpG密度高,不活跃的调节区域.
- 与非甲基化位点的结合涉及其他域或蛋白质-蛋白质相互作用.
- 努RD复合物调解MBD2与非甲基化活性区域的结合.
- 此外,MBD3还独立于基甲基化,结合非甲基化位点.
结论:
- 揭示了MBD蛋白质的明确的甲基化依赖和独立结合模式.
- 修订了MBD蛋白质对DNA甲基化读数的当前模型.
- 提供功能结合图,以了解MBD蛋白在基因调节中的作用.
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