在核酸减少酶β2的结网中发生的与氧化相关的变化
Adam R Offenbacher1, Ellen C Minnihan, JoAnne Stubbe
1Department of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
基尿素 (HU) 触发了核酸减少酶 (RNR) β2亚单元的基本差异性-铁基共同因子的结构变化. 这些变化涉及改变的键,可能调节酶失活路径.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 酶学 是一种酶学.
背景情况:
- рибо核酸减少酶 (RNR) 对于DNA复制和修复至关重要.
- 一级Ia RNRs在β2子单元中使用一个differric-tyrosyl基 (Y122O(•)) 辅因子.
- 氧尿素 (HU) 是一种激素清除剂和与RNR相互作用的癌症治疗剂.
研究的目的:
- 通过HU.研究Y122O降低Escherichia coli β2的机制.
- 测试假设Y122OH氧化还原反应会诱导差异性集群中的结构变化.
主要方法:
- 采用反应诱导的里埃变换红外光谱法 (FT-IR).
- 用[(13) C]阿斯巴酸标记的β2进行同位素分析.
- 分析的Y122O (((•) 减去Y122OH的差异光谱.
主要成果:
- 观察到D84的不对称振动中的频率转移,D84是差异性集群的配体.
- 提供了Y122OH环境中氧化还原诱导变化的证据.
- 表明Y122OH和D84.4之间的结发生了变化.
结论:
- Y122OH氧化还原反应与不同的结构变化有关.
- 氧化还原诱导的键转移可能会调节HU介导的RNR失活中的质子转移.
- 调查结果提供了关于RNR监管和HU行动机制的见解.
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