活细胞的光学磁性成像
1Harvard-Smithsonian Center for Astrophysics, Cambridge, Massachusetts 02138, USA.
Nature
|April 27, 2013
概括
这项研究引入了一种新的磁成像技术,使用钻石空缺中心来以亚细胞分辨率可视化活细胞内的磁纳米粒子. 这一突破使在环境条件下生物磁性结构的详细绘制成为可能.
科学领域:
- 生物物理学的生物物理.
- 量子传感器是一种量子传感器.
- 显微镜的使用方法
背景情况:
- 现有的磁性成像方法既缺乏亚细胞分辨率,也缺乏在环境条件下成像活样本的能力.
- 像MRI这样的技术提供了较差的空间分辨率,而像SQUID显微镜这样的技术则需要极端条件,不适合生物研究.
研究的目的:
- 开发一种适用于环境条件下的生物系统的高分辨率磁性成像技术.
- 为了可视化和表征细胞内磁性结构,如磁性细菌中的磁体体.
主要方法:
- 利用光学检测的磁场成像阵列,基于钻石中的空 (NV) 颜色中心.
- 探测NV量子自旋状态以重建位于钻石芯片上的活细菌中磁体的矢量磁场组件.
- 集成宽场显微镜用于平行光学和磁性成像,分辨率低于微米.
主要成果:
- 实现了细胞下空间分辨率 (400纳米) 用于活体磁拍性细菌的磁性成像.
- 成功地绘制了细菌磁体链所产生的矢量磁场.
- 空间相关的磁场图与光学图像,使得磁体的精确定位和特征.
结论:
- 在环境条件下在活细胞中展示了高分辨率生物磁性成像的新能力.
- 该技术允许在细胞和蜂网络中绘制磁信号的映射.
- 开辟了研究生物系统中各种磁现象的途径,以前所未有的细节.
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