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Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Published on: August 13, 2013
甲状腺衍生调节性T细胞有助于耐受性对共生微生物群的产生
Anna Cebula1, Michal Seweryn, Grzegorz A Rempala
1Center for Biotechnology and Genomic Medicine, Georgia Regents University, Augusta, Gerogia 30912, USA.
Nature
|April 30, 2013
概括
瘤衍生的调节性T (Treg) 细胞,而不是诱导的Treg细胞,是维持肠道耐受性的关键. 这些Treg细胞受到肠道微生物的影响,防止对共生细菌的免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物组研究的研究.
- T细胞生物学T细胞生物学
背景情况:
- 对自我和共生抗原的外周耐受性依赖于调节性T (Treg) 细胞.
- 特雷格细胞可以起源于胸腺,也可以通过异位诱导.
- 甲状腺衍生细胞与诱导的Treg细胞在肠道平衡中的不同作用仍然不清楚.
研究的目的:
- 为了确定肠道Treg细胞的起源和抗原特异性.
- 研究不同Treg细胞子集对肠道免疫耐受性的贡献.
- 为了澄清维持肠道平衡的机制,以对抗开始性抗原.
主要方法:
- 单细胞和高通量T细胞 CD4 ((+) Foxp3 ((+) 和 CD4 ((+) Foxp3 ((-) T细胞的受体测序.
- 对T细胞针对特定的开始性物种的反应性的分析.
- 在淋巴细胞和肠道器官中对Treg细胞谱的比较分析.
主要成果:
- 胸腺衍生Treg细胞是包括结肠在内的所有淋巴状和肠道器官中占主导地位的Treg细胞群.
- 肠道Treg细胞的T细胞受体谱系在很大程度上是由肠道微生物群组成的.
- 诱导的Treg细胞在调解对肠道同类动物的耐受性方面起到很小的作用.
结论:
- 来自胸腺的Treg细胞是对肠道共生抗原的耐受性的主要媒介.
- 肠道微生物群的组成影响了负责维持肠道平衡的Treg细胞的谱.
- 这些发现挑战了关于诱导的Treg细胞在肠道耐受性中占主导地位的先前假设.
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