R-循环稳定抑制了在Arabidopsis FLC位点的反意义转录
Qianwen Sun1, Tibor Csorba, Konstantina Skourti-Stathaki
1Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
概括
家庭主体蛋白质AtNDX通过稳定Arabidopsis FLOWERING LOCUS C (FLC) 促进体中的R环来调节长非编码RNA (lncRNA) COOLAIR转录. 这种机制影响FLC基因表达.
科学领域:
- 分子生物学分子生物学
- 植物遗传学 植物遗传学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 长非编码RNAs (lncRNAs) 在基因表达中发挥作用,但它们自身的调节尚未得到充分理解.
- 反感转录的调节,比如COOLAIR起源于阿拉比多普西斯花C (FLC) 位点,仍然在很大程度上未被探索.
研究的目的:
- 为了确定控制lncRNA表达的调节因素.
- 为了研究家庭主体蛋白AtNDX调节COOLAIR转录的机制.
主要方法:
- 在体外DNA结合测试中,以表征AtNDX与DNA的相互作用.
- 在Arabidopsis基因组内对AtNDX的体内定位研究.
- 在COOLAIR促进体中检测RNA-DNA杂交物 (R-循环).
- 分析FLC基因表达的反应AtNDX介导的R循环稳定.
主要成果:
- 家庭主体蛋白AtNDX被确定为lncRNA COOLAIR的调节者.
- AtNDX与单链DNA结合,并定位到COOLAIR促进体的异色区.
- 在体内,AtNDX稳定了R环,R环是覆盖COOLAIR促进体的RNA-DNA混合体.
- 通过AtNDX稳定R环抑制COOLAIR转录,随后影响FLC表达.
结论:
- 在NDX中介的R循环稳定是一种调节lncRNA转录的新机制.
- 这种调节途径影响了Arabidopsis.中的FLC基因的表达.
- 不同的R循环稳定可能是控制各种生物体基因表达的一般机制.
相关概念视频
Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Transcription Attenuation in Prokaryotes
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...


