发光的穿透细胞的五十核核胺团
Dominique T Thielemann1, Anna T Wagner, Esther Rösch
1Institute of Inorganic Chemistry, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.
Journal of the American Chemical Society
|May 9, 2013
概括
合成了带有类联结体的新型兰坦化氧化集群. 这些集群显示出生物成像的潜力,在细胞溶解体中积累,并在HeLa瘤细胞中表现出中度细胞毒性.
科学领域:
- 无机化学 无机化学 有机化学
- 材料科学 材料科学 材料科学
- 生物医学应用 生物医学应用
背景情况:
- 兰化物团因其独特的磁性和发光性质而引起人们的兴趣.
- 开发新的配体对于稳定和功能化这些集群至关重要.
- 类提供了一个多功能平台,用于设计功能性连接体.
研究的目的:
- 通过使用类联体合成和表征一种新型的五度核胺酸氧集群.
- 在溶液中调查集群的结构完整性.
- 评估合成集群的发光特性和体外生物活性.
主要方法:
- 单晶X射线晶体学用于固态结构的确定.
- 电子喷雾电离质谱仪 (ESI-MS) 用于溶液研究.
- 对焦显微镜 (TRLLM模式) 用于细胞吸收研究.
- 在 HeLa 瘤细胞的体外细胞毒性测试中.
主要成果:
- 一个新型的五核二核胺氧化集群[{Ln15(μ3-OH) 20 ((PepCO2) 10 ((DBM) 10Cl}Cl4]被成功制备和表征.
- 集群核心在溶液中保持完整,正如ESI-MS所证明的那样.
- 基于欧的集群 (1) 呈现出强烈的红色光 (11%的量子产量),而基于的集群 (2) 则显示出较弱的发射.
- 在HeLa细胞的内体 - 解体系统中积聚的.
- 这两个都对HeLa瘤细胞表现出中度的细胞毒性.
结论:
- 类的成功结合作为支持连接体在兰化聚类中,为功能材料开辟了新的途径.
- 发光特性和细胞吸收机制表明生物成像中的潜在应用.
- 中度细胞毒性需要进一步研究治疗应用.
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