相关实验视频

Updated: May 11, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
11:08

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

用工程酶和功能化辅因子替代物标记氨酸转化酶基质

Chao Yang1, Jiaqi Mi, You Feng

  • 1Department of Chemistry, Georgia State University, P.O. Box 4098, Atlanta, Georgia 30302-4098, USA.

Journal of the American Chemical Society
|May 11, 2013
PubMed
概括

研究人员开发了化学探针,以识别蛋白质氨酸转化酶 (KAT) 标. 这种方法使用工程化KAT和合成乙-CoA替代物来精确标记和检测细胞中的KAT基质.

更多相关视频

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues12:07

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

相关概念视频

Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...