在10亿年的进化过程中,bZIP蛋白与蛋白相互作用的网络多样化了
Aaron W Reinke1, Jiyeon Baek, Orr Ashenberg
1Massachusetts Institute of Technology, Department of Biology, Cambridge, MA 02139, USA.
Science (New York, N.Y.)
|May 11, 2013
概括
物种多样性源于不断演变的蛋白质相互作用. 基本区域-氨酸拉链 (bZIP) 转录因子网络显示,与单细胞生物体相比,多细胞生物体的复杂性和重新连接增加,这是由生物分子序列和功能的变化所驱动的.
科学领域:
- 分子生物学分子生物学
- 进化生物学 进化生物学
- 基因组学就是基因组学.
背景情况:
- 生物分子序列和功能的差异推动了物种在外观和行为上的多样性.
- 转录因子,如基本区域-氨酸拉链 (bZIP) 蛋白质,在调节基因表达中起着至关重要的作用,是理解进化适应的关键.
研究的目的:
- 量化和比较bZIP转录因子的二分化网络在各种物种之间,包括甲基动物和单细胞生物.
- 研究bZIP互动体内的进化变化和网络复杂性以及它们对物种多样性的贡献.
主要方法:
- 进行了体外相互作用试验,以测量bZIP因子的2891对蛋白质之间的二分化.
- 对bZIP二分化网络进行了对五种甲状动物和两种单细胞物种的比较分析.
主要成果:
- 与单细胞物种相比,metazoan bZIP网络具有更高的复杂性和更高比例的异构相互作用.
- 虽然甲动物的bZIP交互体具有结构上的相似性,但自最后一个共同祖先以来,已经发生了重要的连接重新连接,从而形成了独特的特定物种网络.
- 在bZIP正义和对义的微小序列变化可以导致相互作用特异性的实质性差异.
结论:
- bZIP二元化网络的演变,以广泛的重新布线和改变的相互作用特点为特征,对元动物生命的多样性作出了重大贡献.
- 信号传递和基因表达中的生化功能转移,由这些不断演变的蛋白质相互作用介导,是物种多样化的基本驱动力.
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