铁3-x) Ti-x) O4纳米颗粒作为微生物金属氧化的可调试探头
Juan Liu1, Carolyn I Pearce, Chongxuan Liu
1Pacific Northwest National Laboratory, Richland, Washington 99352, USA. juan.liu@pnnl.gov
Journal of the American Chemical Society
|May 16, 2013
概括
研究人员量化了细菌和氧化铁纳米粒子之间的电子转移. 他们发现,较高的铁比率增加了电子转移,揭示了纳米材料转换的关键微生物途径.
科学领域:
- 生物地质化学生物地质化学
- 纳米材料科学 纳米材料科学
- 微生物的新陈代谢
背景情况:
- 微生物-纳米材料相互作用对于生物技术应用和铁 (氧化) 氧化物纳米材料的环境命运至关重要.
- 鉴于系统的复杂性,在微生物-纳米材料接口的电子转移量化具有挑战性.
研究的目的:
- 单独和表征一种特定的分子路径,用于氧化铁 (oxyhydr) 氧化物纳米颗粒的细菌酶.
- 为了研究纳米粒子组成对电子转移动学的影响.
主要方法:
- 使用量身定制的Fe3-x) Ti-x) O4 (磁铁-铁磁铁) 纳米粒子,具有不同的Ti-IV) 兴奋剂,以控制Fe-II) /Fe-III) 比率.
- 作为电子转移酶,采用了细菌的十海姆c型细胞染色体MtoA.
- 应用在现场的X射线衍射和Fe L(2,3) 边缘的X射线磁圆二元化,用于结构和化学分析.
主要成果:
- 从纳米粒子到MtoA的电子转移动力学系统地增加,Fe (II) /Fe (III) 比率更高.
- 在氧化过程中,螺旋铁晶格在氧化过程中保持不变,结构Fe (II) 的逐渐耗尽.
- 证明MtoA可以在纳米粒子接口的B子网上直接访问Fe (II).
结论:
- 这项研究为铁 (氧化) 氧化物纳米材料的生物转化提供了对一个孤立的分子途径的第一个定量见解.
- 展示了用于探测微生物-纳米材料界面电子转移的新技术,使用量身定制的纳米粒子,纯化的酶和同步子光谱学.
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