磁光发光光开关 - - 在DNA检测中具有双重模式
Eric D Smolensky1, Katie L Peterson, Evan A Weitz
1Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Journal of the American Chemical Society
|May 23, 2013
概括
新的响应磁发光铁氧化物纳米粒子提供使用MRI和发光的DNA的双检测. 这些纳米粒子具有独特的"捕获释放"特性,可用于潜在的DNA净化应用.
科学领域:
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
背景情况:
- 开发用于敏感和特定生物检测的新型纳米材料至关重要.
- 磁光发光纳米粒子为多模式成像和诊断提供了潜力.
- 响应剂在目标结合时改变属性是非常可取的.
研究的目的:
- 为了合成和表征两个新型响应磁发光铁氧化物纳米粒子.
- 用MRI和发光光谱学来评估它们对DNA的双模式检测能力.
- 通过可逆捕捉释放机制探索它们的DNA净化潜力.
主要方法:
- 铁氧化物纳米粒子与聚乙烯糖醇 (PEG) 连接器和金属间接器结合的合成.
- 对两种金属间隔器的研究:Ru (bpy) (phen) (dppz) (发光) 和Eu-DOTA-Phen (发光关闭).
- 评估磁发光特性,DNA结合反应,以及DNA相互作用前后的MRI放松性.
主要成果:
- 合成了17-20纳米磁铁芯,配有PEG涂层,具有高效的MRI对比度.
- 经过DNA插入后,证明了显著的发光变化 (Ru增加20倍,Eu灭>95%).
- 观察到MRI放松度 (r1和r2) 的变化,这是由于纳米粒子在DNA结合时聚集在一起.
结论:
- 开发的磁发光纳米粒子作为有效的双模式DNA传感器起作用.
- 它们的响应性和MRI对比特性适合敏感检测.
- 这种可逆捕获释放能力表明了DNA净化和分离的潜在应用.
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