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相关概念视频

The Extracellular Matrix01:42

The Extracellular Matrix

Overview
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...

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相关实验视频

Updated: May 11, 2026

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

一种分泌的二硫化物催化剂控制细胞外矩阵的组成和功能.

Tal Ilani1, Assaf Alon, Iris Grossman

  • 1Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel. tal.ilani@weizmann.ac.il

Science (New York, N.Y.)
|May 25, 2013
PubMed
概括
此摘要是机器生成的。

酸硫氧化酶1 (QSOX1) 对于将拉米因纳入细胞外基质 (ECM) 至关重要. 没有QSOX1,ECM是有缺陷的,损害细胞粘附和迁移.

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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Published on: May 2, 2019

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

相关实验视频

Last Updated: May 11, 2026

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

科学领域:

  • 生物化学 生物化学
  • 细胞生物学 细胞生物学
  • 细胞外矩阵生物学 细胞外矩阵生物学

背景情况:

  • 在分泌蛋白中形成二硫化键通常发生在内质网膜 (ER) 中.
  • 奎斯硫氧化酶1 (QSOX1) 是一种不寻常的二硫化催化剂,在戈尔吉装置中发现或分泌.
  • QSOX1的细胞外功能在很大程度上仍未被描述.

研究的目的:

  • 为了研究细胞外素硫氧化酶1 (QSOX1) 在 de novo 二硫化键形成中的生理作用.
  • 确定QSOX1对细胞外基质 (ECM) 组成和功能的影响.
  • 评估QSOX1抑制对诸如粘附和迁移等细胞过程的影响.

主要方法:

  • 利用生物化学分析来测量QSOX1在细胞外环境中的活性.
  • 产生并使用单克隆抗体来抑制QSOX1功能.
  • 分析了由具有和没有QSOX1活性的纤维细胞合成的细胞外基质 (ECM) 的组成.
  • 通过功能测试评估细胞矩阵粘附和细胞迁移.

主要成果:

  • QSOX1的活性对于拉米宁正确地纳入纤维细胞合成的ECM至关重要.
  • 缺乏QSOX1活性的ECM在支持细胞矩阵粘附方面表现出缺陷.
  • 使用单克隆抗体抑制QSOX1,改变了ECM特性,减少了细胞迁移.

结论:

  • 细胞外QSOX1在ECM组装和完整性中起着至关重要的作用.
  • 通过QSOX1介导的二硫化键形成对依赖ECM的细胞粘附和迁移至关重要.
  • 准QSOX1提供了一个潜在的策略来调节ECM特性和细胞行为.