对于小鼠的反应的细胞和电路
Santosh K Mishra1, Mark A Hoon
1Molecular Genetics Unit, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research/NIH, 49 Convent Drive, Bethesda, MD 20892, USA.
概括
研究人员确定了尿性聚乙烯 (Nppb) 作为关键的神经在的神经元途径. Nppb会引发,而它在小鼠中的缺席会显著减少的反应.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 皮肤病学 皮肤病学
背景情况:
- () 主要由体感神经元介导,这些神经元表达过渡受体潜在通道亚家族V成员1 (TRPV1) 的短暂受体.
- 精确的分子机制和控制感的神经元通路尚未完全阐明.
研究的目的:
- 为了研究神经在TRPV1表达神经元中中介的作用.
- 为了确定特定的神经和神经元途径参与信号传递.
主要方法:
- 使用Nppb淘汰 (Nppb(-/-)) 鼠标来评估对刺激的行为反应.
- 在野外类型和Nppb(-/-) 小鼠中注射内Nppb.
- 采用毒素介导除来消除Nppb受体表达细胞,并测试对其他神经的反应.
主要成果:
- Nppb在TRPV1神经元的一个子集中表达.
- Nppb(-/-) 小鼠对诱发的药物表现出显著的行为反应减少.
- 内Nppb注射诱导了强烈的伤行为.
- 对Nppb受体表达细胞的毒素中介切除阻断了Nppb诱导的,但没有对释放的反应.
结论:
- 性多乙烯 (Nppb) 被确定为一种关键的选择性神经.
- Nppb作用于TRPV1神经元的下游,引起的感觉.
- 这项研究定义了一个专门用于的神经元通路,涉及Nppb及其受体,其次是其他信号分子.
相关概念视频
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