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相关概念视频

Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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相关实验视频

Updated: May 10, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

选择性核酸捕获与屏蔽的共价探头.

Jeffrey R Vieregg1, Hosea M Nelson, Brian M Stoltz

  • 1Department of Bioengineering, California Institute of Technology, Pasadena, California 91125, USA.

Journal of the American Chemical Society
|June 11, 2013
PubMed
概括

屏蔽共价 (SC) 探头通过将基配对与共价交叉连接相结合,提供增强的选择性和持久的核酸捕获. 这些探针克服了传统方法的局限性,即使在破坏性条件下也能实现稳定的结合.

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 核酸化学的核酸化学

背景情况:

  • 传统的核酸探针依赖于基配对,导致选择性和亲和性的限制.
  • 不完美的选择性和可逆结合阻碍了传统探针的效率和稳定性.
  • 探针选择性和目标分子捕获的耐用性之间存在着一个基本的权衡.

研究的目的:

  • 引入和描述防护共价 (SC) 探头,克服传统核酸探头的局限性.
  • 证明SC探头能够实现高选择性和持久的目标捕获的能力.
  • 探索SC探针在各种生物环境中的潜在应用.

主要方法:

  • 开发使用可编程基配对和分子构造变化的SC探针,以实现选择性.
  • 整合可激活的共价交叉链接,用于持久的目标捕获.
  • 用DNA/RNA寡核酸和全长mRNA点在各种条件下测试探头性能.

主要成果:

  • SC探测器实现了对互补的DNA/RNA点的近定量共价捕获,对不匹配的点进行了高度歧视 (2-3级).
  • 对于全长mRNA标,被证明有选择性的共价捕获,可与寡核酸捕获相提并论.
  • 单核酸不匹配被有效地拒绝 (1-2 个数量级的歧视),交叉链接是可逆的.

更多相关视频

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
10:44

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

相关实验视频

Last Updated: May 10, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow
10:44

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

结论:

  • SC探测器成功地将高序列选择性与持久的目标保留相结合,即使在变质条件下.
  • 这种新的探测器化学克服了选择性和稳定结合之间的传统权衡.
  • 在具有挑战性的环境中,SC探针可用于选择性和稳定的核酸检测.