结合甲型流感病毒H7N9血凝素的葡萄糖受体
Kannan Tharakaraman1, Akila Jayaraman, Rahul Raman
1Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Infectious Diseases Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Cell
|June 11, 2013
概括
H7N9型流感病毒对人类受体的结合性有限,但单一突变可能会在上呼吸道中实现广泛的结合. 这一发现对潜在的流行病传播和疫苗的有效性有影响.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 公共卫生 公共卫生
背景情况:
- 2013年出现的H7N9流感菌株,由于人类的传染性和缺乏先前存在的免疫力,造成了严重的公共卫生问题.
- 以前的研究表明,H7N9血凝素 (HA) 中的人类受体结合和抗原变化的潜力.
研究的目的:
- 为了研究H7N9血凝素 (HA) 的人类受体结合能力.
- 评估潜在突变对HA结合亲和力和抗原性质的影响.
- 评估对H7N9大流行潜力和疫苗有效性的影响.
主要方法:
- 对H7N9血凝素 (HA) 基因的序列分析.
- 在模型中预测结构变化和受体结合.
- 在抗原区域中共同演变的氨基酸的分析.
主要成果:
- H7N9 HA对人类受体具有有限的结合.
- 一个单一的氨基酸突变可以显著增强HA与上呼吸道人类受体的结合.
- 在HA的抗原区域中确定了共进化的氨基酸,这表明了潜在的抗原漂移.
结论:
- H7N9病毒具有通过特定突变适应人类有效传播的潜力.
- 在HA的抗原变化可能会影响当前的疫情前H7疫苗的有效性.
- 进一步的监测和研究对于监测H7N9的演变和疫情准备至关重要.
相关概念视频
Influenza
Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...
Glycocalyx and its Functions
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
Components of...
Components of...
Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...


