这个消息将自毁:NMD调节轴突引导
Nicolas Preitner1, Jie Quan, John G Flanagan
1Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA.
Cell
|June 11, 2013
概括
调节的轴突内蛋白质合成和无意义介导的mRNA衰变 (NMD) 控制Robo3.2表达. 这种分子开关对于引导轴突在神经发育过程中到达正确的目的地至关重要.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 轴导航是一个复杂的过程,对于形成功能神经电路至关重要.
- 轴指导依赖于一系列分子线索和受体相互作用.
- 了解引导受体的调节是解读神经发育的关键.
研究的目的:
- 调查轴突内蛋白质合成在轴突指导中的作用.
- 阐明控制导航过程中指导受体表达的机制.
- 为了确定参与调节Robo3.2功能的分子参与者.
主要方法:
- 对轴突内蛋白质合成的分析.
- 对无意义介导的mRNA衰变 (NMD) 途径的调查.
- 关于Robo3.2表达动态的研究.
- 阿克森指导测定体内和体外.
主要成果:
- 证明受调节的轴突内蛋白质合成对于轴突路径发现至关重要.
- 展示了无意中介mRNA衰变 (NMD) 在控制Robo3.2表达中的关键作用.
- 在NMD介导的Robo3.2表达中发现了一个交换机,这对于导航至关重要.
结论:
- 内轴突蛋白质合成与NMD相结合,提供了轴突指导的调节机制.
- 这种机制允许引导受体表达的动态变化,从而实现精确的导航.
- 这些发现为神经电路形成的分子控制提供了新的见解.
相关概念视频
02:27Nonsense-mediated mRNA Decay
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
02:27Nonsense-mediated mRNA Decay
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
01:15Neurogenesis and Regeneration of Nervous Tissue
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
01:09Synaptic Signaling
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
01:12Synaptic Signaling
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.