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An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
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通过有氧糖溶解对T细胞效应因子功能的转录后控制

Chih-Hao Chang1, Jonathan D Curtis, Leonard B Maggi

  • 1Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Cell
|June 11, 2013
PubMed
概括

细胞使用有氧糖解不是用于增殖,而是用于效应器功能,如IFN-γ生产. 这种代谢开关由GAPDH与IFN-γmRNA结合来调节,控制细胞因子的产生.

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What is Glycolysis?00:56

What is Glycolysis?

Overview
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Nearly all the energy used by cells comes from the bonds that make up complex organic compounds. These organic compounds are broken down into simpler molecules, such as glucose. As a result, cells extract energy from glucose over many chemical reactions—a process called cellular respiration.
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Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
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Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...
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