ZFP36L2对于早期爆发形成单元红色素原始体的自我更新是必要的
Lingbo Zhang1, Lina Prak, Violeta Rayon-Estrada
1Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
Nature
|June 11, 2013
概括
结合RNA的蛋白质ZFP36L2对于红细胞的产生至关重要. 它充当分子开关,维持早期祖先的自我更新 (BFU-E) 并使红色素细胞生成增加.
科学领域:
- 血液形成 血液形成 血液形成
- 分子生物学分子生物学
- 细胞调节 细胞调节 细胞调节
背景情况:
- 各种血统中的干细胞和祖细胞通过自我更新分裂,这是由未知的蛋白质调节的过程.
- 葡萄糖皮质类药物通过促进早期爆发形成单元-红色球体 (BFU-E) 的祖先的自我更新来增强红细胞的形成.
研究的目的:
- 为了确定调节干细胞自我更新的蛋白质在红状腺血统.
- 为了研究ZFP36L2在葡萄糖皮质激素诱导的BFU-E自我更新和红色素形成中的作用.
主要方法:
- 研究ZFP36L2作为BFU-Es.的葡萄糖皮质体受体 (GR) 的转录标.
- 在培养的BFU-E细胞和移植的红色素原体中进行了ZFP36L2的淘汰实验.
- 在红细胞分化过程中分析了ZFP36L2与信使RNA (mRNA) 的结合.
主要成果:
- ZFP36L2是GR的转录标,对于BFU-E自我更新至关重要.
- 葡萄糖皮质体激动剂维持ZFP36L2的表达,这通常是在红细胞分化过程中下调.
- ZFP36L2 Knockdown 干扰了葡萄糖皮质激素诱导的BFU-E自我更新,并阻止了体内红色素原体的扩张.
- ZFP36L2与末端红色球分化过程中升级的mRNAs结合,从而负调节它们的表达.
结论:
- ZFP36L2作为一个分子开关,促进BFU-E自我更新.
- 这种自我更新会导致殖民地形成单位-红色球体 (CFU-E) 先生的数量增加和整体红色球体细胞的产生.
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