新的功能柄用于纳米医学中作为自我报告的对比剂和输送剂
Mathew P Robin1, Anne B Mabire, Joanne C Damborsky
1Department of Chemistry, University of Warwick, Library Road, Coventry CV4 7AL, United Kingdom.
Journal of the American Chemical Society
|June 15, 2013
概括
研究人员开发了一种使用dithiomaleimide (DTM) 进行先进成像的新型发射聚合物系统. 该系统自行报告微粒分解,从而能够精确跟踪生物环境中的颗粒降解.
科学领域:
- 聚合物化学 聚合物化学
- 材料科学 材料科学 材料科学
- 生物医学成像技术 生物医学成像技术
背景情况:
- 块共聚合物对于自组装到用于药物输送和对比剂的核心外结构至关重要.
- 开发能够无集成到聚合物架构中的新型发射功能对于先进的成像应用是必不可少的.
- 迪胺 (DTM) 为聚合物合并提供了一个小型,高排放和多功能功能组.
研究的目的:
- 合成和描述一种使用dithiomaleimide (DTM) 的新型染色体桥梁块共聚合物系统.
- 评估基于DTM的区块共聚物的光物理性质和自我组装行为.
- 展示该系统在生物背景下对菌细胞解体的定量成像和跟踪方面的潜力.
主要方法:
- 合成一个含有dithiomaleimide (DTM) 功能组的双块共聚物.
- 光物理特征包括在广泛的度范围内进行排放和异性质测量.
- 时间域光终身成像 (FLIM) 来解决超分子状态的差异,并监测小细胞分解.
- 在实验室应用研究中,使用FLIM观察大鼠海马组织中的小细胞分解.
主要成果:
- 基于DTM的区块共聚合物与自由染料相比,具有增强的排放,具有独立于度的光物理性质.
- 该系统形成稳定的米塞尔,不会自我灭,保持高排放.
- FLIM成功地解决了区块共聚合物微粒的超分子状态的差异.
- 在拆卸过程中,DTM 菌体表现出自报告能力,可通过 FLIM 观察.
- 试验室内实验表明,该系统能够追踪生物组织中的小细胞分解.
结论:
- 具有DTM功能的区块共聚合物代表了一种适用于定量成像的新类链内发射器.
- 该系统能够自我报告拆卸的能力使其在跟踪生物环境中的粒子降解方面具有价值.
- 这种新材料具有先进的体外诊断和成像应用的巨大潜力.
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