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一个线性数组α-氨基酸的折叠在一个螺旋式芳香的橄胺框架内

Mayumi Kudo¹, Victor Maurizot, Brice Kauffmann

¹Department of Chemistry, Faculty of Science, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.

Journal of the American Chemical Society

|June 15, 2013

概括

研究人员开发了一种新的方法,使用螺旋式基胺物线线性地排列蛋白质构建块. 这创造了有序的氨基酸侧链阵列,用于设计具有特定识别能力的蛋白质模仿.

科学领域:

超分子化学超分子化学
有机化学有机化学
生物材料科学生物材料科学

背景情况:

控制氨基酸侧链的空间布局对于创建蛋白质模拟器至关重要.
对目标蛋白表面的选择性识别需要这些侧链的精确空间组织.

研究的目的:

提出一种新的方法来产生蛋白质原残留物线性阵列.
为控制空间组织,将α-氨基酸纳入螺旋式基胺序列.

主要方法:

将L-氨酸 (L) 纳入8 - 氨基-2-氨酸碳酸酸 (Q) 二元体 (Q2) 的序列中.
在溶液中使用循环二极化 (CD) 光谱分析螺旋形状的分析.
通过X射线晶体学进行固态结构的确定.
在溶液中使用核磁共振 (NMR) 谱法进行对形分析.

主要成果:

L-(LQ2) n 序列采用了右侧螺旋形状.
每个LQ2段形成了一个螺旋旋转,距离3.5 Å.
在 (LQ2)4.4中形成了一个由四个白氨酸侧链组成的线性阵列.
核磁共振揭示了溶液中两个缓慢平衡的折叠合体的混合物,其比例取决于溶剂.

结论:

一种新的方法使得在螺旋状的橄胺结构中能够创建氨基酸侧链的线性数组.
这种有序排列的侧链对于开发具有选择性结合性质的蛋白质模仿物至关重要.
这些结构的结构性行为对溶剂条件敏感,为可调节的分子识别提供了潜力.

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