相关实验视频
Updated: Feb 16, 2026
08:31
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
Published on: July 9, 2019
7.7K
通过驱逐CTCFF,Jpx RNA激活了Xist,从而激活了Xist
Sha Sun1, Brian C Del Rosario, Attila Szanto
1Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.
Cell
|June 25, 2013
概括
X染色体不活化 (XCI) 是通过分子定位机制来调节的. JpxRNA与CTCF蛋白结合,将其从Xist基因中释放出来,在哺乳动物中启动XCI.
科学领域:
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 哺乳动物的剂量补偿依赖于XX个体的X染色体失活 (XCI).
- 非编码的XistRNA启动XCI,但其精确的调节仍然不清楚.
- 现有的模型表明X-to-autosome比率 (X:A) 的依赖性,缺乏详细的分子见解.
研究的目的:
- 阐明控制XistRNA诱导和XCI启动的分子因素.
- 研究RNA和蛋白质在调节Xist转录中的相互作用.
- 了解CTCF在XCI监管中的作用机制.
主要方法:
- 研究了X编码RNA和自体编码蛋白之间的分子定位.
- 分析了CTCF蛋白在XCI前细胞中抑制Xist转录中的作用.
- 研究了JpxRNA上调对CTCF与Xist等位基因结合的影响.
主要成果:
- 证明JpxRNA,一个X编码的分子,在XCI开始时升级.
- 表明JpxRNA结合自体因子CTCF蛋白,并将其从Xist促进体释放出来.
- 已证实CTCF是一种由JpxRNA定位的RNA结合蛋白,从而激活Xist转录.
结论:
- 晶状体诱导是由JpxRNA和CTCF蛋白之间的分子定位决定的.
- 通过对抗CTCF介导的抑制,JpxRNA激活了Xist.
- 这种机制突显了长非编码RNAs在通过RNA-蛋白相互作用的表观遗传调节中的作用.
更多相关视频
10:10HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
Published on: March 31, 2019
8.8K
12:42Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
Published on: November 26, 2014
14.7K
相关概念视频
00:58Eukaryotic RNA Polymerases
27.2K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.2K
01:53RNA Stability
35.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K
01:23RNA Interference
28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
01:23RNA Structure
79.3K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.3K
02:18Alternative RNA Splicing
25.3K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.3K
01:32RNA Splicing
60.8K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.8K