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哺乳动物细胞中的DNA计算:微RNA逻辑操作
James Hemphill1, Alexander Deiters
1Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, United States.
Journal of the American Chemical Society
|June 26, 2013
概括
科学家们设计了DNA逻辑门,可以检测哺乳动物细胞中的特定微RNA (miRNA). 这些分子计算机使细胞特异性检测和对miRNA变化的反应成为可能,为生物应用推进DNA计算.
科学领域:
- 分子生物学分子生物学
- 合成生物学 合成生物学
- 生物技术是生物技术.
背景情况:
- DNA计算使用逻辑门来为具有生物输入的分子计算机进行计算.
- 带有核酸输入的模块化电路通过链杂交激活计算级联.
- 合成电路可以设计为与细胞环境的接口.
研究的目的:
- 在活哺乳动物细胞中设计寡核酸和特定微RNA (miRNA) 检测门.
- 开发基于单一和双传感miRNA的计算设备,用于细胞特异性内源miRNAs的鉴定.
- 为了证明细胞环境中核酸逻辑门的功能,以识别内源输入.
主要方法:
- 工程寡核酸和门对特定的miRNA输入有反应.
- 合成单个和双感应miRNA基于计算设备.
- 在活哺乳动物细胞中测试设备,以识别内源性miR-21和miR-122.
- 观察逻辑门对miRNA表达调节器和配置变化的反应.
主要成果:
- 在哺乳动物细胞中成功设计了响应特定miRNA输入的AND门.
- 开发并测试了用于内源性miRNA检测的单个和双传感器件.
- 通过逻辑门响应证明了miRNA特征变化的分子识别.
- 在细胞环境中证实了核酸逻辑门的功能.
结论:
- 工程核酸逻辑门在细胞环境中具有功能,并识别内源输入.
- 这项工作显著扩大了DNA计算的潜力,用于监测,成像和响应细胞特异性标记物.
- 合成生物学电路为精确的生物检测和响应机制提供了新的可能性.
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

