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Updated: May 10, 2026

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

实时观察多种蛋白质复合体的形成,使用单分子FRET

Wooli Bae, Woori Bae1, Mal-Gi Choi

  • 1National Creative Research Initiative Center for Single-Molecule Systems Biology and Department of Physics, KAIST, Daejeon 305-701, South Korea.

Journal of the American Chemical Society
|July 2, 2013
PubMed
概括

新的单分子FRET方法允许实时观察多种蛋白质相互作用. 这揭示了可溶性N-乙基胺胺敏感因子附着蛋白受体 (SNARE) 复合体的合作形成,这表明了级联机制.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...